Longitudinal Profiling of the Tissue-Specific Expression of Genes Related with Insulin Sensitivity in Dairy Cows during Lactation Focusing on Different Fat Depots

نویسندگان

  • Behnam Saremi
  • Sarah Winand
  • Paula Friedrichs
  • Asako Kinoshita
  • Jürgen Rehage
  • Sven Dänicke
  • Susanne Häussler
  • Gerhard Breves
  • Manfred Mielenz
  • Helga Sauerwein
چکیده

In dairy cows the milk associated energy output in early lactation exceeds the input via voluntary feed intake. To spare glucose for mammary lactose synthesis, peripheral insulin sensitivity (IS) is reduced and fat mobilization is stimulated. For these processes a link between IS and the endocrine functions of adipose tissue (AT) is likely; we thus aimed to characterise the mRNA expression from bovine AT derived proteins and receptors that are related to IS according to the literature in metabolically active tissues plus systemic IS throughout lactation. Conjugated linoleic acids (CLA) reduce milk fat thus decreasing the milk drain of energy and potentially dampening lipolysis, but may also affect IS. Subcutaneous (s.c.) AT and liver from pluriparous cows receiving either control fat or CLA supplement (100 g/day from 1 to 182 days in milk each) were biopsied covering week -3 to 36 relative to parturition. In an additional trial with primiparous cows treated analogously and slaughtered on days in milk 1, 42 or 105, samples from liver, udder, skeletal muscle and 3 visceral and 3 s.c. AT were obtained and assayed for mRNA abundance of adiponectin, its receptors, leptin, leptin receptor, PPARγ, PPARγ2, IL-6, and TNF-α. In pluriparous animals, the mRNA abundance of most of the target genes decreased after parturition in s.c. AT but increased in liver. In primiparous cows, AT depot specific differences were mostly related to retroperitoneal AT; adiponectin receptor 1 and TNF-α were affected predominantly. CLA effects in primiparous cows were largely limited to decreased PPARγ2 mRNA abundance in udder tissue. In pluriparous cows, insulin secretion was increased by CLA resulting in decreased systemic IS but without consistent changes in tissue target mRNA abundance. The temporal gene expression profiles from the adipokines and related receptors support their coactive function in adapting to the needs of lactation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Response of lactating dairy ruminant to different profiles of dietary calcium soaps of fatty acids

Nutrition has a significant effect on reproduction and lactation in Ruminant. Supply of require nutrient of dairy cows and ewes in early lactation is the main challenges that will be considered, since in most cases the animals facing negative energy balance after calving and the beginning of the period of lactation. Negative energy balance may sometimes result in reduced livestock production an...

متن کامل

Response of lactating dairy ruminant to different profiles of dietary calcium soaps of fatty acids

Nutrition has a significant effect on reproduction and lactation in Ruminant. Supply of require nutrient of dairy cows and ewes in early lactation is the main challenges that will be considered, since in most cases the animals facing negative energy balance after calving and the beginning of the period of lactation. Negative energy balance may sometimes result in reduced livestock production an...

متن کامل

Response of lactating dairy ruminant to different profiles of dietary calcium soaps of fatty acids

Nutrition has a significant effect on reproduction and lactation in Ruminant. Supply of require nutrient of dairy cows and ewes in early lactation is the main challenges that will be considered, since in most cases the animals facing negative energy balance after calving and the beginning of the period of lactation. Negative energy balance may sometimes result in reduced livestock production an...

متن کامل

Response of lactating dairy ruminant to different profiles of dietary calcium soaps of fatty acids

Nutrition has a significant effect on reproduction and lactation in Ruminant. Supply of require nutrient of dairy cows and ewes in early lactation is the main challenges that will be considered, since in most cases the animals facing negative energy balance after calving and the beginning of the period of lactation. Negative energy balance may sometimes result in reduced livestock production an...

متن کامل

Validation of Reference Genes for Real Time PCR Normalization in Milk Somatic Cells of Holstein Dairy Cattle

Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 9  شماره 

صفحات  -

تاریخ انتشار 2014